Reaction mechanism of chitosanase from Streptomyces sp. N174

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Reaction mechanism of chitosanase from Streptomyces sp. N174.

Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of the products were analysed by NMR spectroscopy. The chitosanase produced heterooligosaccharides consisting of D-G...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1995

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj3110377